Vitek 2 automated identification system and kocuria kristinae article pdf available in journal of clinical microbiology 4311. Us environmental protection agency office of pesticide programs. Microbiological identification and antibiotic susceptibility testing system. A preinserted transfer straw means less manual steps. The reliability of using vitek 2 compact system to detect. Pdf vitek 2 automated identification system and kocuria. Use, maintenance and quality control procedures scope the purpose of this sop is to describe the procedures for the. The configuration that you have purchased is adapted to the legislation. Quality control for quality control purposes, the required information is documented on the appropriate forms see section 14.
We amplified and sequenced gene fragments of glutamate dehydrogenase and recombinationrepair protein and conducted pcr serotyping to confirm some serotypes. Vitek 2 is a unique system that uses a phenotypic expert system instead of commonly used. Manufacturer specifications vitek 2 compact, biomerieux please note. The vitek 2 system correctly identified to the species level only one choice or after simple supplementary tests 99% of s. Advances in life science and technology issn 22247181 paper issn 2225062x online vol. The automated system guarantees excellence and optimizes your workflow. Vitek 2 compact is designed for medium to smallsized laboratories which require an automated system that will cover the majority of their routine tests. The vitek 2 is an automated microbiology system utilizing growthbased technology.
Principles the vitek 2 is an automated microbiology system utilizing growthbased technology. The vitek 2 anaerobe and corynebacterium anc identification card is a newly developed method for identification of. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Gram positif menggunakan metode manual dan otomatik, sehingga dapat diketahui. A broad choice of methods is available, based on both phenotypic and molecular properties of microbes.
The existing vitek 2 60 and 120 xl systems are designed for large microbiology laboratories requiring high test throughput. The refurbished biomerieux vitek 2 is a fully automated system that. The system is available in three formats vitek 2 compact, vitek 2, and vitek 2 xl that differ in increasing levels of capacity and automation. Application product type product name product description product number. In diagnostic microbiology as well as in microbiological research, the identification of a microorganism is a crucial and decisive stage. Malditof ms versus vitek 2 anc card for identification of. Ensure highperformance products, reproducible clinical test results and consistent microbiological evaluation of pharmaceuticals and food with thermo scientific cultiloops quality control organisms. Safety is optimized with minimal reagent preparation and reduced handling in a closed, disposable system.
Vitek 2 is a fully automated system that performs bacterial identification and antibiotic susceptibility testing vitek 2 offers. Features of the biomerieux vitek 2 gram negative susceptibility test cards. The product information manual, software user manual, industry customer training course book and instrument user manual are maintained near the instrument. In our study, vitek 2 and phoenix showed similar performance for most bacterial groups except for nfgnb which showed relatively low identification rate.
Application using colorimetric reagent cards, the vitek. Vitek 2 compact instrument dimensions mass height 60 cm 23. Vitek 2 uses unique id and ast cards the size and shape of a playing card. Vitek susceptibility test results are expressed as minimum inhibitory concentration mic values and interpreted as susceptible, intermediate or resistant by reference to a clsi. Vitek 2 compact identification cards gp, bcl, and gn. Evaluation of the vitek 2 system for identification and. Tenovera adivision of healthcare quality promotion, centers for disease control and prevention, atlanta, georgia, usa brollins school of public health, emory university, atlanta, georgia, usa abstract we tested 143 isolates of. Used biomerieux vitek2 compact microbiology analyzer for. Use of the vitek 2 system for rapid identification of. Ergonomic analysis comparison of the vitek 2 and vitek 2 compact. Modifications by biomerieux can be made without prior notice biomerieux, the blue logo, advanced expert system, bioart, densichek, myla, vilink and vitek are used, pending andor registered trademarks belonging to biomerieux, or one of its subsid iaries, or one of its. It provides rapid, automatic, standardized validation of every test result with next generation expert software, the advanced. Sixtyfour well iuo cards were then loaded into the.
To inoculate the vitek2 cards directly from a positive blood culture bottle, a 5ml aliquot was centrifuged at 60 g for 10 min. The quiz enables you to measure your level of proficiency on the routine use of the software. Inoculate organism into 3ml vitek saline tube, vortex and invert the tube 2 to 3 times to uniformly suspend inoculum. The refurbished biomerieux vitek 2 compact idast system has everything laboratories need for fast, accurate microbial identification and susceptibility testing. Agreement and cost comparison of vitek2 compact, vitek2 60, vitek ms systems and api20ene using clinical gramnegative bacilli isolates from jamaica september 20 doi. Pdf agreement and cost comparison of vitek2 compact. Save configuration vitek cameras save the configurations after every step. The product is used with the vitek 2 automated system which consists of instruments, software and reagent cards designed for the identification and antimicrobial susceptibility testing of bacteria and yeast. Vitek 2 compact, automated idast instrument biomerieux.
Performance of vitek2 compact and overnight microscan. Building construction, power outages and equipment maintenance may cause transient aberrant counts. Employing the logic that the vitek will only accept up to a 6 digit accession number, and no accession shall exceed 99,999. Vitek 2 xl vitek 2 compact vitek 2 0118 93192002gbe document andor pictures not legally binding. Fast, accurate, resistanceoriented susceptibility test results. Designed to provide idast results in as little as 5 to 8 hours3, vitek 2 compact works with economical, readytouse vitek 2 idast cards. After primary organism isolation, there is minimal. Description of the biomerieux vitek 2 gram negative susceptibility test cards, astgn04, 20pk. Biomerieux vitek 2 gram negative susceptibility test cards. Volume 25, number 12december 2019 emerging infectious. User interface screen for immediate notification of system status to increase productivity unique vacuum filler provides both safety and the highest level of automation. Biomerieux inc 595 anglum rd hazelwood mo 630422320. Vitek 2 compact identifies the vast majority of microorganisms that contaminate. The reliability of using vitek 2 compact system to detect 1.
Class 2 device recall biomerieux vitek 2 systems software. Available in two sizes, the refurbished biomerieux vitek 2 compact is easy to set up and operate and it features advanced colorimetry identification technology to enhance performance. The aim of this study was to compare the application of phenotypic and molecular tools in bacterial identification on the example of. These organisms may be automatically transferred to this area of the software and saved. All identification stages from reading to the recording of results are automated, optimizing workflow. All isolates were tested with gram negative susceptibility. Vitek manual table of contents mount sinai hospital. The prefix number could be placed in front of the 5 digit accession. The vitek 2 compact system biomerieux,france was used for confirmation of. Matrixassisted laser desorption ionization timeofflight mass spectrometry malditof ms is an accurate, rapid and inexpensive technique that has initiated a revolution in the clinical microbiology laboratory for identification of pathogens. Vitek2 compact system direct testing procedure idgn and astn 020 cards were used, respectively, for identification and susceptibility testing directly from positive bactalert fa and fn blood cultures. The data is representative of performance on both the vitek 2 and vitek 2 compact instrument platforms, as evidenced in the ast equivalency study presented in the vitek 2 compact file, k050002. It is a fully automated system that eliminates the inherent bias of manual mic determination.
Us environmental protection agency office of pesticide. Subculture frozen or freeze dried isolates twice before setting up any vitek 2 cards. Used biomerieux vitek 2 microbiology for sale dotmed. Available with 15, 30, or 60card capacity to meet any labs needs. Densichek meter with mcfarland standards for calibration see section 15. Thermo scientific vitek 2 qc sets include a readytouse set of qc organisms in cultiloops for. Srf, supplemental react file, a customizable feature to track facilityspecific organisms. Vitek 2 technology represents a smarter way to automate idast testing. Your browser does not currently recognize any of the video formats available. The user is advised to read and understand all instructions in this manual to be able to derive the best performance from the vitek 2 instrument and the smart carrier station. Manufacturer specifications vitek 2 compact, biomerieux. Vitek 2 instrument user manual i 51073110en1 general warnings important. The reliability of using vitek 2 compact system to detect extendedspectrum betalactamaseproducing isolates in escherichia coli and klebsiella pneumoniae in accra, ghana.
Vitek 2 compact vtc, vitek 2 60 vt60 and api20ene all biomerieux use biochemicals for bacterial identification id, while the vitek ms. The vitek is an automated system for identification and antibiotic susceptibility testing. Place a small barcode label near the top of a 3ml vitek saline tube. Personnel are required to be knowledgeable of the procedures in this sop.
Application of routine diagnostic procedure, vitek 2. Used microbiological culture analyzer biomerieux vitek 2. Evaluation of new colorimetric vitek 2 yeast identification card by use of different source media article pdf available in journal of clinical microbiology 4611. Roberts, 2014 the highest variability is observed for piperacillin.
In each line, log data consists of date, time, category, ip address, user id. The innovative vitek 2 microbial identification system includes an expanded identification database, the most automated platform available, rapid results, improved confidence, with minimal. Vitek 2 and phoenix have been reported to yield the same or similar high identification results for nonfermenters, staphylococci, enterococci, and enterobacteriaceae eigner et al. We conducted microbiological assays using the fully automatic vitek 2 compact system. The system is available in three formats vitek 2 compact, vitek 2, and vitek 2 xl that differ in increasing levels of. As the system operates with barcoded cards, full traceability is ensured and the risk of transcription errors is minimized.